The biocontrol of stem blight of asparagus Phomosis asparagi was proved by
Neosartorya and Talaromyces. The symptoms are appearance as oval-shaped lesions with light
brown centres and slightly darker margins. Ten isolates of pathogen were isolated by tissue
transplanting technique and confirmed by morphology and molecular identification based on
internal transcribed spacer (ITS)-nrDNA sequence as P. asparagi. Fungal mycelia showed
undulate margins and colonies white to gray and reached a diameter of 9 cm petri dish after 10
days. Alpha conidia were oblong or spindle-shaped, with a size of 2‒3.5 × 6.5‒8.8 μm. The
fungal pathogenicity test showed the asparagus was infected by P. asparagi within 2 days on
moisture chamber at room temperature. Neosartorya and Talaromyces were proved antifungal
acrtivety against P. asparagi causing of stem blight of asparagus in dual culture. It revealed that
N. hiratsuka EU06 was the highest inhibition P. asparagi causing the stem blight of asparagus
at 59.50%.
Keywords
Stem blight, Phomosis asparagi, Neosartorya and Talaromyces
INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
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