INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY

Volume 14, No. 07, Month DECEMBER, Year 2018, Pages 1379 - 1390

Genetic relationship of maternal lineages in phetchaburi native cattle

Laosutthipong, C. and Chuawongboon, P.

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Maternally inheritance can be described via mitochondrial DNA (mtDNA) analysis for evolution and genetic diversity study in animals and human, especially in cattle. The genetic relationship and cluster analysis of Phetchaburi native cattle in Thailand was investigated. All 7 semen samples collected from male native cattle breeders in sperm bank collection from Phetchaburi province. The mtDNA at D-loop region (467 bp.) was analyzed and the high variable region (288 bp.) was aligned with reference cattle sequences. The results revealed 26 polymorphic sites. The sequence identity of all samples ranged from 96.8 to 99.7% which most of them similar to Bos taurus (AY515627), except MT-1(MH746114) showed 99% identity to Bos indicus (HQ234738). All mtDNA sequences were classified into 3 haplotypes from 7 sperm samples and the haplotype diversity 0.524. The phylogenetic tree analysis indicated 6 breeders were grouped into B. taurus clade whereas only one breeder was located in B. indicus clade. This current results provide the genetic background of native breeder cattle in Phetchaburi province including genetic diversity and breeder grouping which may have implications for breeding management and conservation of Thai native cattle.

Keywords

genetic relationship, maternal lineages, native cattle

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