Neck orange (Citrus reticulate Blanco) is native orange that very popular in southern
of Thailand. It had health benefits and also used in many industries of citrus processing. There
are expansion of the plantation area to increase the production of sufficient to demand of
consumers. The branches are currently not enough to cultivation. This study showed the effect
of benzyladenine (BA) and naphthalene acetic acid (NAA) to develop a new plant of Neck
orange. The seeds of orange were surface sterilized with sodium hypochlorite and culturing on
Murashige and Skoog (MS) medium supplemented with BA (0-2 mg/L) and NAA (0-1 mg/L),
3% sucrose, with and without 0.2% activated charcoal for 6 weeks. The result revealed that MS
free medium (without plant growth regulators) gave the highest percentage of germination
(83.93 percent). The highest of Shoot length (3.17 cm) were obtain from MS medium
containing 0.5 mg/L BA adding activated charcoal, whereas MS medium with 2.0 mg/L BA
gave the highest number of shoot (3.33 shoots/seed) and MS medium with 0.5 mg/L NAA, 0.5
mg/L BA adding activated charcoal gave the highest root length (21.30 cm).