Puberty can be characterized by the time when the cattle first estrus. Estrus in cattle
can be observed through the cytology of the vaginal epithelial (Vaginal smear). The vaginal
wall cytology of Brahman cattle predicted at the onset of puberty was studied. Brahman cattles
were developed by Livestock Breeding Center for Excellence and Forage (BPTU-HPT)
Sembawa since 2012. The estimation of puberty was using 3 nonlinear mathematical models,
there are consisted of Gompertz (10.28 months, n = 4), Bertalanffy (09.96 months, n = 4), and
Logistics (15.5 months, n = 7). Vaginal smear was done by smearing wet cotton bud using
aquadest on Brahman cattle vaginal wall, then it was smeared to glass object. The object glasses
were immersed in alcohol (70%) for 5 to 7 minutes, then it were soaked in liquid Giemsa stain
(5%) for 45 minutes. The object glasses were dried, then it were observed using a microscope
with a magnification 40 times. The vaginal smear samples were taken 8 times and done every 3
days for each cattles. The results described that there were only parabasal and intermediates cell
in all ages of cattle observed. All the animal observation was not entered the age of puberty.
Keywords
Brahman, Estrus, Puberty, Smear, Vagina
INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
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