Efficiency of antioxidant and absorbent on browning and the optimal factors of plant regeneration from young seed of gluta usitata (217 mae ka) by tissue culture
Rakrawee, R., Kittibanpacha, K., Chareonsap, P. and Poeaim, A.
Gluta usitata (Anacardiaceae) is a lacquer tree and create lacquer varnish can be
utilized in many aspects especially art and culture. Recently, its harvest faced troublesome and
yields of varnish tree are decreased. Consequently, the conservation of plant has reduced, and
this plant has become extinct. The experiment was chosen to use plant tissue culture technique
to preserve and increase this plant. The experiment was compared the effect of Woody Plant
Medium (WPM) combined with different concentration of antioxidants were 2, 4, 6, 8 g/L Poly
Vinyl Pyrrolidone (PVP), 20, 30, 40, 60 mg/L ascorbic acid or citric acid and absorbents of 1, 2,
3 g/L activated charcoal to reduce browning of phenolic compound in the explants. The results
showed that 2 g/L activated charcoal gave the lowest of browning on seed and medium (about
0.9±0.2 points). The addition of 40 mg/L ascorbic acid was generated the highest of shoots
(approximately 1.88±0.4 shoots/seed) after culture for 4 weeks. in vitro propagation of seed
germination on WPM supplemented with 0.5, 1, 1.5, 2 and 3 mg/L 6-Benzyl amino purine
(BAP), Thidiazuron (TDZ) and meta-Topolin (mT) 3% sucrose and 0.2% phytagel was
efficiently resulted. Other result revealed that 1.5 mg/L BAP, 2 mg/L TDZ and 2 mg/L mT gave
the highest seed germination at 1.6±0.2, 1.8±0.2 and 1.6±0.2 shoots/seed, respectively. The best
of plant growth regulators for seed germination was 2 mg/L of TDZ after 6 weeks.
Keywords
Absorbent, Antioxidant, Browning, Gluta usitata (217 Mae Ka), Plant regeneration
INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY
Published by : Association of Agricultural Technology in Southeast Asia (AATSEA) Contributions welcome at : http://www.ijat-aatsea.com
By using our website, you acknowledge that you have read and understand our Cookie Policy and Privacy Policy.