Kadsura spp. is a vine glabrous woody which belongs to Schisandraceae that is
rare plant found in highland. It is an ancient plant group that low adaptation for living.
Shape and scent of fruits like sugar apple, and edible when ripping. It contains the nutrition
value and high antioxidants. It is a valuable medicine to prevent the tumor HIV resistant
and hepatitis. In presently Kadsura spp. become extinct. The calli from leaves of Kadsura
spp. was induced by this study. The leaves were sterilized and cultured on solid synthetic
medium, Murashige and Skoog (MS) medium supplement with Plant Growth Regulators
(PGRs) were used as 0.5, 1, 2, 3 and 5 mg/L concentrations of 6-benzylaminopurine (BAP),
meta-Topolin (mT), 2, 4-dichlorophenol-xyacetic acid (2,4-D) and 0.5 mg/L of BAP
combined with 0.5, 1, 2, 3 and 5 mg/L of 2,4-D. The maximum number of leaves were
induced calli of 55.55 % and 350.16 mm3
averaged area on medium with 0.5 mg/L of BAP
with 0.5 mg/L of 2,4-D, but 0.5 mg/L of BAP with 2 mg/L of 2,4-D gave the highest
average area (1,079.53 mm3
) after 4 weeks. The growth rate of cell suspension cultured in
liquid MS medium supplement with 0.5 mg/l of BAP and 0.5 mg/l of 2,4-D resulted the
fresh weight and dry weight of cell suspension with the best grown for 15 days of 1.12 g/10
mL, 0.14 g/10 mL fresh and dry weight respectively. Cell suspension was rapidly grown
during the period of 6-15 days. The cell suspension was cultured on MS solid medium
combine with 0.5 mg/l of BAP and 0.5 mg/l of 2,4-D and 0.2% (W/V) AC after 4 weeks.
The best result for calli induction and perfectly for plant regeneration were the MS medium
without AC. This research work is developed an optimized protocol for plant breeding.