INTERNATIONAL JOURNAL OF AGRICULTURAL TECHNOLOGY

Volume 14, No. 06, Month NOVEMBER, Year 2018, Pages 833 - 844

Biosynthesis of zinc nanoparticles and its effect on enzymes production by bacillus subtilis and pseudomonus flourescens using different agricultural wastes.

El-Mohamedy, R. S. R., El-Gamal, N. G., El-Shamy, A. R. and Atalla S. M. M.

Abstract Download PDF

Biological synthesis of zinc nanoparticles is an environmental eco-friendly.Bacillus subtilis and Pseudomonas fluorescens which has been isolated from healthy beans plants and identified in plant pathology department at ( NRC ) were used for production of pectinase and chitinase enzymes in media containing different agriculture wastes and zinc nitrate to initiate nanoparticles synthesis. Pomegranate peel and Colocasia peel were the most promising wastes in zinc nanoparticles synthesis 100, 200 nm as proven by Transmission electron microscopy (TEM) and UV visible spectral analysis. The effectiveness of different agricultural wastes on the linear growth of green bean root rot fungi in vitro was investigated. Also, Pomegranate peel and Colocasia peel were the most effective on reducing linear growth of all pathogens. And enzymes activity from two bacterial strains was used different agricultural wastes as substrate and its antagonistic effects on pathogenic fungi.

Keywords

B.subtilis, P.fluorescens, zinc nanoparticles, enzymes, Agricultural wastes

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