ENGINEERING JOURNAL CHIANG MAI UNIVERSITY

Volume 27, No. 02, Month AUGUST, Year 2020, Pages 216 - 226

Design and fabrication of microfluidic chip for charged beads separation using gel electrodes

Chalinee Phiphattanaphiphop, Rungrueang Phatthanakun and Komgrit Leksakul

Abstract Download PDF

This research presents a new model for the design and fabrication of a gel electrode microfluidic device for charged beads separating with an electrophoresis electric field. In order to achieve more efficient beads separation, the use of this gel electrode to help solve the problem of the occurrence of oxide / metal chemicals used to make the electrode when it comes into contact with the fluid. And to prevent certain substances that have been exposed to corrosion or chemical reactions to the electrode, and finally, to prevent heat generation which may result in the flow channel having bubbles which will hinder the flow of the sample in the flow channel, which will cause a lot of obstacles in separating the particles. Therefore, this research presents designing and fabrication of an electrode using gel electrodes for particle separation applications for further use in medicine. By the designing of structure was created and designed with the layout@ program. Then the microelectrode device was fabricated with a gel electrode by using lithography techniques and soft- lithography techniques for fabrication the microchannel for flow the beads into the microchannel. Finally, the testing was set up for the separation of charged particles in gel electrode microfluidic device by power supply 1 voltage. It was found that the structure can precisely separate the charged particles with the accuracy percentage of the particles with 82.18% negative charge and the positive charged particles at 79.92% respectively at the 95% confidence level.

Keywords

Microfluidic chip, charged beads separation, gel electrodes

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