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ENGINEERING JOURNAL CHIANG MAI UNIVERSITY


Volume 26, No. 02, Month AUGUST, Year 2019, Pages 179 - 189


Appropriate parameters of plasma activated water for growth inhibition of e. coli by box-behnken design

Settawut Janthima, Choncharoen Sawangrat, Dheerawan Boonyawan, Saisamorn Lumyong, Jatorong Kumla and Nakarin Suwannarach


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The objective of this study were to find appropriate parameters of Plasma Activated Water (PAW) for hydroxyl radical (OH•) and growth inhibition of microorganisms. Box-Behnken design (BBD) was applied to determine parameter of factors in experiment. The independent variables were expose time, argon gas and oxygen mixture while hydroxyl radical and E. coli reduction were dependent variables. As a result, the optimal parameter were expose time 20 minute, argon gas flow rate 5 L/min and oxygen mixture 2% of Ar gas. The optimal parameters leads to approximately 10-30 ppm of hydroxyl radical and 0.62-0.98 (70-80%) Log CFU/ml of E. coli reduction. In addition, the optimal parameters was used to treat with chili and can reduce approximately 0.69, 0.72 and 0.82 (70-80%) Log CFU/g of microorganisms with treatment time 5, 10 and 15 minute, respectively.


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ENGINEERING JOURNAL CHIANG MAI UNIVERSITY


Published by : Faculty of Engineering Chiang Mai University
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