The objective of this study were to find appropriate parameters of Plasma Activated Water (PAW) for hydroxyl radical (OH•) and growth inhibition of microorganisms. Box-Behnken design (BBD) was applied to determine parameter of factors in experiment. The independent variables were
expose time, argon gas and oxygen mixture while hydroxyl radical and E. coli reduction were dependent variables. As a result, the optimal parameter were expose time 20 minute, argon gas flow rate 5 L/min and oxygen mixture 2% of Ar gas. The optimal parameters leads to approximately 10-30 ppm of hydroxyl radical and 0.62-0.98 (70-80%) Log CFU/ml of E. coli reduction. In addition, the optimal parameters was used to treat with chili and can reduce approximately 0.69, 0.72 and 0.82 (70-80%) Log CFU/g of microorganisms with treatment time 5, 10 and 15 minute, respectively.