This research studied ethanol production from algae cultivated by waste water from processing
and product development plant of Royal Project Foundation. Ethanol productions from 4 strains of algae including Pediastrum sp. AARL G060 , Chlorella sp. AARL G049 , Microalgal consortium and
Scenedesmus sp.AARL G085 were studied along with their production cost and economic value. The
results showed that the microalgal consortium was the most suitable raw material for ethanol production.
Steam explosion at 45 psi for 50 minutes combined with enzymaic digestion by cellulase with
concentration of 2.00 ml per 1 g of algae at 50 ° C for 70 hours resulted in 20.25 mg/ml of reducing
sugar, 21.36 mg/ml of total sugar content and 1.10 mg/ml of nonreducing sugar. In addition, the optimal
condition for ethanol production from algae was 1.30 % Saccharomyces cerevisiae using, incubated at
pH 6.71 and room temperature for 8 days. The resulting ethanol concentration was 7.17%. When
increase the amount of Microalgal consortium to 400 g, it was found that ethanol production from the
pretreated algae under optimal conditions had ethanol concentration, reducing sugar and volumes of
liquids of 7.30%, 1.15 mg / ml and 3,289 ml, respectivetly. The cost of ethanol production from algae
was 39.08 baht per liter with simple payback period of 4.77 years and 21% of return on investment.
Keywords
Ethanol, Algae Cultivation, Waste Water, Vegetable and Fruit Processing Plant