This study aimed to optimize the extraction method for placenta which gave the
highest biological content and activity using a central composite face-centered experimental
design model. The effects of temperature and pH were selected to investigate for 2 responses,
the ABTS scavenging activity and total protein on placenta extraction. Then, the optimized
method was implemented to compare the wound healing effects on fibroblast cell migration
and endothelial cell tube formation between porcine and Aries placenta extracts. The optimal
extraction method which gave the highest trolox equivalent and total protein content was pH
9.07 and at 50°C. This optimal extraction condition gave the non-significant biological contents
and activities of porcine and Aries placenta extracts. The Aries placenta extract gave higher total
protein content and ABTS scavenging activity, but lower elastase and collagenase inhibition
activity than those of porcine extract. Both Aries and porcine placenta extracts gave higher
percentages of wound closure, cell migration rate and significantly longer in the capillary length
than those of the standard L-ascorbic acid. Both placenta extracts from the optimized method
showed potential effects on wound healing on fibroblast cell migration and endothelial cell tube
formation which can be studied for the further wound healing investigation.