CHIANG MAI JOURNAL OF SCIENCEVolume 46, No. 02, Month MARCH, Year 2019, Pages 196 - 206
Enhanced growth and ethanol oxidation by overexpressed caia gene encoding acyl-coa dehydrogenase in komagataeibacter medellinensis nbrc 3288
Phisurang Konjanda, Toshiharu Yakushi, Kazunobu Matsushita and Gunjana Theeragool
Abstract Download PDFIn this study, the caiA gene encoding acyl-CoA dehydrogenase (ACAD) from
thermotolerant Komagataeibacter intermedius MSKU 9 was cloned and characterized. Two specific
primers for amplification of an entire caiA gene were designed from a draft genome sequence
of K. intermedius MSKU 9 (MSKU 9_0337 homolog). Sequencing analysis of the 1.2 kb PCR
product revealed possible open reading frame (ORF) of 1,182 bp with a start codon ATG at
the 1,001st - 1,003rd position and a stop codon TAA at the 2,183th - 2,185th position. The deduced
amino acid sequences of the caiA gene had 393 residues. The overexpression of the caiA gene
in a non-thermotolerant strain, K. medellinensis NBRC 3288, was investigated. K. medellinensis
NBRC 3288 harboring pMVcaiA showed a better growth rate than that observed in the parental
strain at 33°C and was more tolerant to both ethanol at 30°C and acetic acid even at 37°C.
Thus, it can be suggested that the caiA gene influenced and improved growth, acetic acid
tolerance and ethanol oxidation in acetic acid bacteria at high temperature.
Komagataeibacter, caiA gene, acyl-CoA dehydrogenase, acetic acid tolerance, ethanol oxidation