CHIANG MAI JOURNAL OF SCIENCE
Volume 46, No. 02, Month MARCH, Year 2019, Pages 180 - 195
Pharmaceutical and cosmeceutical biological activities of hemp (cannabis sativa l var. sativa) leaf and seed extracts
Aranya Manosroi, Charinya Chankhampan, Bang-on Kietthanakorn, Warintorn Ruksiriwanich, Puxvadee Chaikul, Korawinwich Boonpisuttinant, Mathukorn Sainakham, Worapaka Manosroi, Theeraphong Tangjai and Jiradej Manosroi
The fresh Hemp (Cannabis sativa L var. sativa) leaves and seeds were extracted by
maceration in 95% ethanol and investigated for their biological activities. The percentage yields
were 3.77±0.31 and 1.58±0.20%, respectively. For cosmeceutical activities, the leaf extract
exhibited higher DPPH scavenging, metal chelating and tyrosinase inhibition activity than
the seed extract. The leaf extract exhibited pro MMP-2 inhibition (56.27±5.98%) and active
MMP-2 inhibition (100%) with the pro MMP-2 inhibition lower than ascorbic acid of 1.11
times. The melanin induction in B16F10 melanoma cells of the leaf extract was 1.06 and 2.22
times of the control and theophylline, respectively. Moreover, it showed 5α-reductase inhibition
(13.57±3.21%), but lower than dutasteride of 3.78 times. For pharmaceutical activities, the
leaf extract showed anti-bacterial activity on S. mutans which was lower than erythromycin
of 17 times and anti-proliferation on HepG2 and KB cell lines (IC50 values=13.17±1.53 and
5.16±1.66 mg/ml), which were lower than doxorubicin of 2,800 and 3,000 time, respectively.
The high apoptotic on HeLa cells of the leaf extract was observed at 13.26±7.40%. The seed
extract showed lipid peroxidation inhibition and anti-bacterial activity on S. aureus higher than
the leaf extract. This study has demonstrated the potential of the Hemp leaf extract which can
be further developed for pharmaceutical and cosmeceutical application.
Keywords
Cannabis sativa L, Hemp, antioxidant, tyrosinase, cytotoxicity, MMP-2, melanin, 5?-reductase, antibacterial, antiproliferative, apoptosis